MAL31, a sugar transporter involved in pullulan biosynthesis in Aureobasidium pullulans
Xing Chen1, Ying Wang1, Xin-Tong Zhang1
1School of Biology and Basic Medical Sciences, Soochow University, 199# Ren'ai Road, Suzhou 215123, People's Republic of China.
Journal of Biotechnology
|October 15, 2022
Summary
The sugar transporter MAL31 significantly impacts pullulan biosynthesis in Aureobasidium pullulans. Modifying MAL31 levels offers a strategy for enhanced polysaccharide production.
Area of Science:
- Biotechnology
- Microbial Physiology
- Biochemistry
Background:
- Pullulan, a polysaccharide produced by Aureobasidium pullulans, has diverse industrial applications.
- Understanding the genetic and metabolic factors regulating pullulan biosynthesis is crucial for optimizing its production.
Purpose of the Study:
- To investigate the specific role of the sugar transporter MAL31 in the biosynthesis of pullulan.
- To determine the effects of MAL31 gene disruption and overexpression on pullulan production in A. pullulans.
Main Methods:
- Genetic manipulation of A. pullulans CCTCC M 2012259 to create mal31-disrupted (A. pullulans Δmal31) and overexpressing (A. pullulans Mal31) strains.
- Batch fermentation experiments to measure pullulan production.
- Kinetics analysis, enzyme assays, and measurement of intracellular metabolites (UDPG, NADH, ATP).
- Transcriptional analysis of genes involved in pullulan biosynthesis and excretion.
Main Results:
- Disruption of mal31 led to a 69.1% decrease in pullulan production and reduced glucose consumption.
- Overexpression of mal31 increased pullulan production by 15.9%, enhanced glucose consumption, and upregulated genes for pullulan biosynthesis.
- Mal31 disruption increased intracellular UDP-glucose (UDPG) for beta-glucan accumulation, indicating a metabolic shift.
Conclusions:
- MAL31 plays a critical role in the transport of precursors essential for pullulan biosynthesis.
- Genetic modification of MAL31 presents a viable strategy for improving pullulan yield in A. pullulans.
- This study provides insights for enhancing the production of other microbial polysaccharides.
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