High-performance liquid chromatographic determination of norfloxacin in human tissues and plasma with fluorescence...

A high-performance liquid chromatographic method with fluorimetric detection is described for the quantitative determination of norfloxacin in renal and prostatic tissues and in plasma. It consists of tissue pretreatment, purification by solid-state extraction and separation and quantification by high-performance liquid chromatography on a C8 reversed-phase column. Analytical recoveries ranged from 95.2 to 97.6%. Within-day and between-day precision were assessed by analysing serum containing 50 and 500 ng/ml norfloxacin. At each concentration, the within-day precision was less than or equal to 3.6% (coefficient of variation; n = 10) and the day-to-day precision was less than or equal to 5.3% (n = 10). The limit of detection was 1 ng/ml.