Hydrogen sulfide-releasing polyurethane/gelatin/keratin-TA conjugate mats for wound healing

Hydrogen sulfide (H₂S) has indispensable effects on wound healing promotion, such as anti-inflammatory, cell proliferation enhancement, and angiogenesis improvement. However, small-molecular donors have drawbacks of burst release and low biocompatibility. In this study, a novel bio-macromolecular donor of H₂S based on a keratin-thiobenzoic acid conjugate (KTC) was first synthesized by a thiol-disulfide exchange reaction. Then, the KTC was incorporated into polyurethane and gelatin to fabricate nanofibrous mats by electrospinning. Interestingly, both the KTC donor and biocomposite mats were capable of releasing H₂S triggered by glutathione (GSH) under physiological conditions. These mats had excellent water absorption capacity, demonstrating their great ability to absorb tissue exudate. In addition, these mats possessed a notable antioxidant ability, thereby protecting cell membranes from oxidation. Cell assays in vitro demonstrated that these mats could promote cell proliferation and migration. Furthermore, the in vivo full-thickness defect model verified that these H₂S-releasing wound dressing mats could accelerate granulation tissue formation, enhance collagen deposition, and promote angiogenesis. Based on these advantages, these wound dressing mats had great potential applications for wound healing.