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Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Spatially Reprogramed Receptor Organization to Switch Cell Behavior Using a DNA Origami-Templated Aptamer Nanoarray.

Miao Wang¹, Donglei Yang², Qin Lu³

¹State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, Hunan 410082, P. R. China.

|October 18, 2022

Summary

We developed a DNA nanoplatform to precisely control receptor tyrosine kinase (RTK) oligomerization, enabling new insights into cell signaling. This tool offers robust control over cellular behaviors by regulating RTK activation.

Keywords:

Aptamer ligand Cellular behavior DNA origami Multivalency Receptor oligomerization

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Area of Science:

Molecular Biology
Nanotechnology
Cell Signaling

Background:

Receptor oligomerization is crucial for cell signaling but lacks precise molecular interrogation tools.
Understanding how receptor oligomerization governs signaling responses is a significant challenge.

Purpose of the Study:

To develop a novel DNA origami-templated aptamer nanoarray (DOTA) for precise control over receptor tyrosine kinase (RTK) oligomerization.
To investigate the impact of defined valency, distribution, and stoichiometry on RTK signaling.
To demonstrate the utility of DOTA in regulating cellular behaviors.

Main Methods:

Fabrication of DNA origami-templated aptamer nanoarrays (DOTA).
Programming RTK oligomerization using monomeric (mALs) and dimeric (dALs) aptamer ligands.
Assessing RTK signaling activation and cellular response (epithelial-to-mesenchymal transition).

Main Results:

DOTA precisely controls RTK oligomerization with defined valency, distribution, and stoichiometry.
Multivalency and nanoscale spacing of RTK oligomers significantly influence signaling activation.
DOTA-modulated RTK oligomerization can switch cell behavior, promoting epithelial-to-mesenchymal transition.

Conclusions:

DOTA is a versatile DNA nanoplatform for systematic investigation and regulation of receptor-mediated cellular responses.
Precise control over RTK oligomerization offers a powerful method to study and manipulate cell signaling.
This technology opens new avenues for understanding and controlling complex cellular processes.