Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Calibration Curves: Linear Least Squares

Calibration Curves: Linear Least Squares

A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...

Calibration Curves: Correlation Coefficient

Calibration Curves: Correlation Coefficient

In a linear calibration curve, there is a value called the calibration coefficient, denoted by 'r,' which measures the strength and the direction of association between two variables. The correlation coefficient value ranges from −1 to +1. A value of +1 indicates a perfect positive linear correlation, −1 denotes a perfect negative correlation, and 0 implies no correlation between the two variables. A positive correlation value establishes that as one variable increases, the...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CIST: A clade-informed sequence transformer framework for predicting influenza virus antigenicity.

Scientific reports·2026

Same author

Magnetic hydrogel-mediated periodic stimulation enhances mesenchymal stem cell small extracellular vesicle release.

Materials today. Bio·2026

Same author

ProSSF: integrating sequence, structure, and gene ontology for prediction of protein stability, interaction, and function.

Molecular genetics and genomics : MGG·2026

Same author

Electroactive hydrogel for force-to-electricity conversion: Emerging engineered frameworks for advanced bio-interfaces.

Biomaterials advances·2026

Same author

Design and implementation of a microscale thermophoresis system based on complementary metal-oxide-semiconductor (CMOS) detector array.

The Review of scientific instruments·2026

Same author

Robust 3D Pose estimation and Parkinson's Disease classification via Dual-Stage Adaptive Temporal Perception and graph topology modeling network.

PloS one·2026

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 25, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Calibration method for a structured light system based on DIC.

Canyu Zhu, Qingchuan Zhang

|October 18, 2022

Summary

This summary is machine-generated.

This study introduces a novel calibration method for structured light 3D shape measurement systems. It enhances accuracy and efficiency for systems using diffractive optical elements (DOEs) and digital image correlation (DIC).

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Published on: January 24, 2016

Related Experiment Videos

Last Updated: Aug 25, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Published on: January 24, 2016

Area of Science:

Metrology and Measurement Science
Optical Engineering
Computer Vision

Background:

Structured light (SL) is a key non-contact 3D shape measurement technique.
Diffractive optical element (DOE)-based SL systems offer cost-effectiveness and compactness.
Existing DOE-based SL systems lack dynamic content projection, preventing phase-shift calibration.

Purpose of the Study:

To propose a novel calibration method for structured light systems, particularly DOE-based ones.
To enable calibration for SL systems that cannot change projected patterns.
To improve calibration efficiency and accuracy compared to existing methods.

Main Methods:

Developed a calibration approach combining the pseudo-camera method with digital image correlation (DIC).
The method is versatile, applicable to both time-sequence coded and DOE-based (speckle) SL systems.
Validation involved experimental measurements of a cylinder within a defined field of view.

Main Results:

Achieved a reprojection error of 0.68 pixels during calibration.
Demonstrated a radius error of less than 1% in the 3D measurement of a cylinder.
The method requires fewer images, no datum plane, and simpler operation than traditional techniques.

Conclusions:

The proposed pseudo-camera and DIC-based calibration method is effective for various SL systems.
It offers significant advantages in terms of simplicity, efficiency, and accuracy.
This advancement facilitates more accessible and precise 3D shape measurement using DOE-based structured light.