Molecular hydrogen positively influences lateral root formation by regulating hydrogen peroxide signaling

Although a previous study discovered that exogenous molecular hydrogen (H₂) supplied with hydrogen-rich water (HRW) can mediate lateral root (LR) development, whether or how endogenous H₂ influences LR formation is still elusive. In this report, mimicking the induction responses in tomato seedlings achieved by HRW or exogenous hydrogen peroxide (H₂O₂; a positive control), transgenic Arabidopsis that overexpressed the hydrogenase1 gene (CrHYD1) from Chlamydomonas reinhardtii not only stimulated endogenous hydrogen peroxide (H₂O₂) production, but also markedly promoted LR formation. Above H₂ and H₂O₂ responses were abolished by the removal of endogenous H₂O₂. Moreover, the changes in transcriptional patterns of representative cell cycle genes and auxin signaling-related genes during LR development in both tomato and transgenic Arabidopsis thaliana matched with above phenotypes. The alternations in the levels of GUS transcripts driven by the CYCB1 promoter and expression of PIN1 protein further indicated that H₂O₂ synthesis was tightly linked to LR formation achieved by endogenous H₂, and cell cycle regulation and auxin-dependent pathway might be their targets. There results might provide a reference for molecular mechanism underlying the regulation of root morphogenesis by H₂.