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Related Experiment Video

Updated: Aug 25, 2025

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

A method for single particle tracking through a multimode fiber.

Angel Cifuentes, Johanna Trägårdh

|October 19, 2022

Summary

We developed a new particle tracking method for multimode optical fiber (MMF) endoscopes, enabling faster imaging deep within tissue. This technique achieves relevant speeds and high accuracy for microscopy applications.

Area of Science:

Biomedical optics
Microscopy
Optical imaging

Background:

Multimode optical fiber (MMF) endoscopes are emerging tools for deep tissue imaging.
Current limitations include the lack of fast wide-field fluorescence imaging modalities for MMF endoscopes.

Purpose of the Study:

To present a novel particle tracking method for MMF endoscopes.
To overcome the speed limitations of existing imaging modalities for MMF endoscopes.

Main Methods:

Implemented a discrete orbital particle tracking technique.
Utilized a spatial light modulator (SLM) for wavefront shaping.
Demonstrated tracking of a pinhole mimicking Brownian motion.

Main Results:

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Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Related Experiment Videos

Last Updated: Aug 25, 2025

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Achieved biologically relevant tracking speeds up to 1.2 μm/s.

Demonstrated tracking accuracy of λ/50 for a 0.3 numerical aperture (NA) fiber.

Successfully tracked simulated Brownian motion with diffusion rates up to 0.3 μm²/s.

Conclusions:

The developed particle tracking method enables high-speed, accurate imaging through MMF endoscopes.
This technique addresses a key limitation in MMF endoscope applications.
The method shows potential for advanced microscopy and deep tissue imaging.