Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A method for single particle tracking through a multimode fiber.

Optics express·2022
Same author

Suppression of the non-linear background in a multimode fibre CARS endoscope.

Biomedical optics express·2022
Same author

Isolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopy.

Analytical and bioanalytical chemistry·2021
Same author

Reply to comment on Improving Poor Man's Kramers-Kronig analysis and Kramers-Kronig constrained variational analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2021
Same author

Deep learning-based classification of blue light cystoscopy imaging during transurethral resection of bladder tumors.

Scientific reports·2021
Same author

Leukocyte Activation Profile Assessed by Raman Spectroscopy Helps Diagnosing Infection and Sepsis.

Critical care explorations·2021

Related Experiment Video

Updated: Jan 4, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

35.0K

Label-free CARS microscopy through a multimode fiber endoscope.

Johanna Trägårdh, Tomáš Pikálek, Mojmír Šerý

    Optics Express
    |November 6, 2019
    PubMed
    Summary

    This study demonstrates label-free chemical imaging using multimode fiber endoscopes with coherent anti-Stokes Raman scattering (CARS) microscopy. This technique enables in vivo imaging of biological tissues for potential cancer diagnosis.

    More Related Videos

    Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
    10:35

    Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

    Published on: October 17, 2016

    8.2K
    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.5K

    Related Experiment Videos

    Last Updated: Jan 4, 2026

    High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
    13:49

    High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

    Published on: January 11, 2011

    35.0K
    Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
    10:35

    Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

    Published on: October 17, 2016

    8.2K
    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.5K

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Spectroscopy

    Background:

    • Multimode fibers are emerging as ultra-thin endoscopes for in vivo imaging.
    • Label-free microscopy offers chemical contrast without exogenous agents.

    Purpose of the Study:

    • To extend multimode fiber endoscopy to label-free nonlinear microscopy.
    • To achieve chemical contrast imaging using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope.
    • To explore applications in in situ diagnosis of potentially malignant tissue.

    Main Methods:

    • Utilized a commercial 125 µm diameter, 0.29 NA GRIN fiber.
    • Employed wavefront shaping with a spatial light modulator (SLM) to create scanned foci.
    • Implemented CARS microscopy through the multimode fiber for chemical imaging.
    • Utilized epi-detection for signal collection.

    Main Results:

    • Demonstrated label-free chemical contrast imaging via CARS through a multimode fiber endoscope.
    • Achieved rapid per-pixel integration times as low as 1 ms.
    • Successfully imaged 2 µm polystyrene and 2.5 µm PMMA beads, showcasing chemical selectivity.

    Conclusions:

    • Multimode fiber CARS endoscopy provides a new platform for label-free chemical imaging in vivo.
    • This technique holds promise for instant and in situ diagnosis of biological tissues.
    • The developed method opens new avenues for advanced endoscopic diagnostics.