Related Experiment Video

Updated: Jun 23, 2026

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
10:24

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Published on: February 21, 2013

John squire and endothelial glycocalyx structure: an unfinished story

Kenton P Arkill1, C Charles Michel2, Elizabeth V M Rider3

  • 1School of Medicine, University of Nottingham Biodiscovery Institute, Nottingham, UK. kenton.arkill@nottingham.ac.uk.

Journal of Muscle Research and Cell Motility
|October 19, 2022
PubMed

Insights

This review highlights John Squire's contributions to vascular permeability and endothelial glycocalyx research. His work is reinterpreted to align with current understanding of glycocalyx structure and composition.

Area of Science:

  • Biomedical Science
  • Physiology
  • Cell Biology

Background:

  • John Squire made significant contributions to the fields of muscle physiology and vascular permeability.
  • His research utilized ultrastructural analysis to investigate the endothelial glycocalyx.

Purpose of the Study:

  • To review John Squire's contributions to the vascular permeability field.
  • To re-evaluate his work in light of current understanding of endothelial glycocalyx structure and composition.

Main Methods:

  • Review of John Squire's published works.
  • Ultrastructural analysis of the endothelial glycocalyx.
  • Reinterpretation of existing data in the context of current knowledge.

Main Results:

  • Squire's research advanced the understanding of endothelial glycocalyx.
  • A reinterpretation links his work to current models predicting 6 glycosaminoglycans fibers per syndecan core protein.
  • Endothelial dimerization is proposed as the mechanism for achieving this structure.

Conclusions:

  • John Squire's legacy extends beyond muscle research into vascular permeability.
  • His foundational work on the endothelial glycocalyx provides insights into its structure and function.
  • The proposed model of glycocalyx structure has implications for understanding vascular permeability.

Keywords:
AlbuminGlycosaminoglycanProteoglycanReflection coefficientVascular permeability

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