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Updated: Aug 24, 2025
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
Published on: November 30, 2022
A lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal in live cells and
Zongyuan Luo1, Zhangyan Zhu1, Tingrui Zhang2
1Department of Pharmaceutical Engineering, College of Food and Bioengineering, Xihua University, Chengdu 610039, China. qians@mail.xhu.edu.cn.
Abstract:
The development of a lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal (MGO) in live cells has important implications for associated diseases. Herein, a lysosome-targeting fluorescent probe MGO-Naph-A was designed and synthesized to detect MGO with high selectivity. The probe contained naphthalimide as the fluorescent group, o-phenylenediamine as the MGO recognition group, and morpholine as the lysosome targeting group. This fluorescent probe could detect endogenous and exogenous MGO in living cells by precisely targeting and staining lysosomes. It could also detect MGO in living zebrafish. The results showed that the probe MGO-Naph-A has the potential to visualize MGO in lysosomes.
