Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

21.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.5K
Proteomics01:33

Proteomics

7.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relay Approach: A Convergent Synthesis of Key Fragments en route to (+)-Neosorangicin A.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Single-cell and pseudobulk analyses reveal hidden mitochondrial expression imbalance in gastric cancer.

Frontiers in genetics·2026
Same author

A de novo LDLR mutation in severe familial hypercholesterolemia: case report, functional characterization, and a personalized gene correction strategy exploration.

Frontiers in cardiovascular medicine·2026
Same author

Acute myocardial infarction induces sex-specific, time-dependent remodeling of the gut microbiome and intestinal immune compartment in retired breeder C57BL/6N mice.

Frontiers in microbiomes·2026
Same author

Potential oncogenic role of occult hepatitis B virus pre-S mutations: Activation of Akt/mTOR/Cyclin D1 signaling drives cell cycle dysregulation and proliferation in hepatocellular carcinogenesis.

Genes & diseases·2026
Same author

Establishment of Pseudovirus-Based Reference Materials and Nationwide External Quality Assessment for Arboviruses during the 2025 Chikungunya Outbreak.

Clinical chemistry·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 24, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.6K

MicroRNA Regulatory Network Analysis Using miRNet 2.0.

Le Chang1, Jianguo Xia2,3,4

  • 1Department of Human Genetics, McGill University, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|October 20, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces miRNet 2.0, a tool for analyzing microRNA (miRNA) regulatory networks. It helps researchers understand how miRNAs interact within complex biological systems to influence cell biology.

Keywords:
Gene regulatory networksNetwork analysisSystems biologymiRNAs

More Related Videos

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.2K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K

Related Experiment Videos

Last Updated: Aug 24, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.6K
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.2K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, operating within complex gene regulatory networks.
  • Interpreting miRNA functions is challenging due to their cooperative actions and interactions with other regulatory elements like non-coding RNAs and transcription factors.
  • High-throughput technologies and abundant gene expression data enable comprehensive miRNA functional studies.

Purpose of the Study:

  • To provide practical guidance on utilizing miRNet 2.0 for miRNA regulatory network analysis.
  • To facilitate the gain of functional insights into miRNA-mediated gene regulation.
  • To address the complexities in interpreting miRNA roles in cellular processes.

Main Methods:

  • The chapter details procedures for using miRNet 2.0, a web-based tool for miRNA network analytics.
  • It focuses on practical application and data interpretation within the miRNet 2.0 framework.
  • Utilizes high-throughput experimental approaches and gene expression data.

Main Results:

  • Demonstrates how miRNet 2.0 can be used to analyze intricate miRNA regulatory networks.
  • Provides a framework for understanding cooperative and complex interactions involving miRNAs.
  • Enables deeper functional insights into miRNA roles in cell biology.

Conclusions:

  • miRNet 2.0 is a valuable tool for dissecting miRNA regulatory networks.
  • The platform aids in overcoming challenges associated with miRNA data interpretation.
  • Facilitates a comprehensive understanding of miRNA functions in biological systems.