Related Experiment Video
Updated: Aug 24, 2025
08:47
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
3.2K
EIF3D promoted cervical carcinoma through Warburg effect by interacting with GRP78
Summary
Eukaryotic translation initiation factor 3 subunit D (EIF3D) promotes cervical cancer growth and the Warburg effect. Inhibiting EIF3D may offer a new therapeutic strategy for cervical carcinoma patients.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Cervical cancer is a significant global health issue, ranking third among female cancers worldwide.
- The specific role of eukaryotic translation initiation factor 3 subunit D (EIF3D) in cervical carcinoma progression remains largely unexplored.
- Understanding novel molecular mechanisms is crucial for developing effective therapeutic strategies.
Purpose of the Study:
- To investigate the role of EIF3D in cervical carcinoma cell progression.
- To elucidate the underlying molecular mechanisms, including its interaction with GRP78.
- To evaluate the potential of EIF3D inhibition as a therapeutic target.
Main Methods:
- Analysis of EIF3D mRNA and protein expression in patient samples and cell lines.
- In vitro and in vivo models to study cell growth and the Warburg effect.
- Investigation of the interaction between EIF3D and GRP78.
Main Results:
- EIF3D expression is upregulated in cervical carcinoma tissues and cell lines.
- Higher EIF3D expression correlates with poorer disease-free and overall survival.
- EIF3D promotes cell proliferation and the Warburg effect, partly via interaction with GRP78.
Conclusions:
- EIF3D plays a critical role in promoting cervical carcinoma cell growth and metabolic reprogramming.
- The interaction between EIF3D and GRP78 is a key mechanism in this process.
- Targeting EIF3D presents a promising therapeutic avenue for cervical cancer treatment.
Related Concept Videos
02:38Mitogens and the Cell Cycle
6.6K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
01:24Small GTPases - Ras and Rho
4.1K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.1K
01:20The Retinoblastoma Gene
4.2K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
01:17Intracellular Signaling Affects Focal Adhesions
2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.8K