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Updated: Aug 24, 2025

Detecting Migration and Infiltration of Neutrophils in Mice

Detecting Migration and Infiltration of Neutrophils in Mice

Published on: February 6, 2020

Human neutrophil development and functionality are enabled in a humanized mouse model.

Yunjiang Zheng¹, Esen Sefik¹, John Astle^1,2

¹Department of Immunobiology, Yale University, New Haven, CT 06520.

Proceedings of the National Academy of Sciences of the United States of America

|October 21, 2022

Summary

This summary is machine-generated.

A new MISTRGGR mouse model enhances human neutrophils, enabling in vivo study of the human immune system. These mice show improved neutrophil function and reduced bacterial burden during infection.

Keywords:

bacterial infection humanized mouse innate immunity neutrophils

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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

Area of Science:

Immunology
Genetics
Animal Models

Background:

Humanized mouse models are crucial for studying the human immune system in vivo.
Current models lack mature, functional human neutrophils, limiting research.
Neutrophils play a key role in innate immunity and host defense.

Purpose of the Study:

To develop a novel humanized mouse model with improved human neutrophil reconstitution.
To overcome the limitations of existing models in studying neutrophil function.
To enable in vivo research on human neutrophils in health and disease.

Main Methods:

Generated MISTRGGR mice by replacing mouse granulocyte colony-stimulating factor (G-CSF) with human G-CSF.
Deleted the mouse G-CSF receptor gene in existing MISTRG mice.
Targeted the G-CSF cytokine-receptor axis to enhance neutrophil development.

Main Results:

Dramatically improved reconstitution of mature, circulating human neutrophils.
Achieved significant infiltration of human neutrophils into tissues.
Demonstrated recruitment of functional human neutrophils upon inflammatory and infectious challenges.
Observed reduced bacterial burden in MISTRGGR mice.

Conclusions:

MISTRGGR mice represent a significant advancement in humanized mouse models.
This model allows for the study of human neutrophil function in vivo.
MISTRGGR mice are valuable for investigating human neutrophil roles in health and disease.