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Updated: Aug 24, 2025
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Gut microbiota in gastrointestinal diseases.
Gargi Bhattacharjee1, Khushal Khambhati1, Nisarg Gohil1
1Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, India.
Progress in Molecular Biology and Translational Science
|October 21, 2022
Summary
The gut microbiota, a complex bacterial community, influences host health and disease. Imbalances in this gut ecosystem are linked to various gastrointestinal disorders, highlighting its critical role in disease development.
Area of Science:
- Microbiology
- Gastroenterology
- Human Health
Background:
- The gut microbiota is a dense, co-evolving bacterial population within the host's gastrointestinal tract.
- This complex ecosystem performs vital metabolic and immunological functions essential for maintaining host health.
- Dysbiosis, or an imbalance in gut microbiota composition, is increasingly recognized as a contributor to various diseases.
Purpose of the Study:
- To elucidate the critical role of gut microbiota in the pathogenesis of gastrointestinal diseases.
- To highlight the association between gut microbiota alterations and the development of specific gastrointestinal conditions.
Main Methods:
- Review of existing literature on gut microbiota composition and function.
- Analysis of factors influencing gut microbiota, including diet, stress, and genetics.
- Correlation of microbial dysbiosis with the incidence and progression of gastrointestinal diseases.
Main Results:
- Alterations in the beneficial gut microbiota population are strongly associated with gastrointestinal diseases.
- Specific conditions like irritable bowel syndrome, inflammatory bowel disease, celiac disease, and colorectal cancer are linked to gut dysbiosis.
- Factors such as diet, stress, and genetic variations significantly impact gut microbiota stability.
Conclusions:
- Gut microbiota plays a pivotal role in the pathogenesis of gastrointestinal diseases.
- Maintaining a balanced gut ecosystem is crucial for gastrointestinal health.
- Understanding gut microbiota dynamics is key to developing therapeutic strategies for GI disorders.
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