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3D-Printed Prolamin Scaffolds for Cell-Based Meat Culture
Lingshan Su1,2, Linzhi Jing1, Xianjian Zeng1,2
1Peak Of Excellent-Center Of Health and Food Technology, National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu, 215123, China.
Advanced Materials (Deerfield Beach, Fla.)
|October 22, 2022
Summary
New edible scaffolds made from cereal prolamins, like hordein and secalin mixed with zein, show promise for cultured meat production. These prolamin scaffolds support muscle stem cell growth and differentiation, enabling the creation of tissue-similar cultured meat.
Area of Science:
- Biomaterials Engineering
- Cellular Agriculture
- Food Science
Background:
- Cultivated meat (CM) production requires 3D edible scaffolds for supporting muscle stem cell growth.
- Electrohydrodynamic (EHD) printing offers high-precision fabrication of ultrafine fibrous scaffolds.
- Developing edible EHD-printable inks is crucial but challenging for CM applications.
Purpose of the Study:
- To develop novel, edible prolamin-based inks for EHD printing.
- To fabricate and characterize EHD-printed prolamin scaffolds for cultured meat.
- To evaluate the potential of these scaffolds in supporting muscle cell growth and differentiation.
Main Methods:
- Zein was mixed with hordein (barley) or secalin (rye) to create prolamin-based inks.
- Optimized printing conditions for EHD printing of prolamin scaffolds.
- In vitro cell culture of porcine skeletal muscle satellite cells (PSCs) on scaffolds.
- Assessment of cell adhesion, proliferation, differentiation, and tissue formation.
Main Results:
- Prolamin-based inks (zein/hordein, zein/secalin) exhibited favorable printability for EHD printing.
- Highly ordered, tessellated prolamin scaffolds were successfully fabricated.
- Scaffolds showed good water stability and in vitro degradability with porous surfaces.
- PSCs adhered, proliferated, and differentiated on the scaffolds, forming a tissue-similar CM slice.
Conclusions:
- Prolamin-based scaffolds are printable using EHD technology and suitable for cultured meat.
- These scaffolds support myogenesis and tissue development, demonstrating significant potential for CM production.
