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Updated: Aug 24, 2025

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
Published on: June 20, 2016
Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System
Maritta Schuez1, Tatiana Sandoval-Guzmán2
1Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Abstract:
The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.
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