Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of N-1 phase media optimization as a bioprocess intensification strategy on fed-batch production.

Bioprocess and biosystems engineering·2026

Same author

Targeting Peptidylarginine Deiminases in Neurons and Astrocytes in Central Nervous System Injury-Effects of Pan-PAD Inhibitor Cl-Amidine in an Oxygen-Glucose Deprivation Model of Ischaemia (OGD/R) and LPS Stimulation In Vitro.

International journal of molecular sciences·2026

Same author

EBV BART microRNA Profiles and Host Gene Links in Gastric Cancer.

Viruses·2026

Same author

A Heavy Metal and Trace Element Biomonitoring Study in a Young Cohort (Aged 18-24) in Istanbul, Turkey.

International journal of environmental research and public health·2026

Same author

Chemopreventive Effects of Lycopene in miR-21 Knockout and Wild-Type Pancreatic Cancer Cells.

Scientifica·2026

Same author

Neuroblastoma: Anti-Invasive Effects of Tetracaine In Vitro.

Basic & clinical pharmacology & toxicology·2026

Same journal

Impact of an Artificial Albumin Corona on Surface Charge-Driven Nano-Bio Interactions and Cytotoxicity of Silver Nanoparticles.

ACS omega·2026

Same journal

Structural and Functional Disruption of Thiopurine S‑Methyltransferase by the A80P Variant: A Simulation and Genotyping Study.

ACS omega·2026

Same journal

CRISPR/Cas12a2-Mediated Ultrasensitive Assay for Rapid Detection of H1N1 Influenza Virus RNA.

ACS omega·2026

Same journal

Photocatalytic Treatment of Real Sugar Industry Wastewater Using Lignocellulosic Biomass-Derived Hydrochar/g-CN.

ACS omega·2026

Same journal

Electrochemical Dopamine Biosensor Based on Plant-Derived Peroxidase Immobilized on Titanate Nanowires.

ACS omega·2026

Same journal

Revealing the Effects of Process Parameters on Structural, Thermal, Mechanical, Biodegradation, and Biocompatibility Properties on the Electrospinning of Poly(vinyl alcohol)/Microbial Inulin Nanofibers.

ACS omega·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 24, 2025

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

microRNA 1307 Is a Potential Target for SARS-CoV-2 Infection: An in Vitro Model.

Elif Damla Arisan¹, D Alwyn Dart², Guy H Grant³

¹Gebze Technical University, Institute of Biotechnology, Gebze, Kocaeli 41400, Turkiye.

|October 24, 2022

Summary

This summary is machine-generated.

microRNAs (miRs) are key in SARS-CoV-2 infection. miR-1307-3p, highly expressed in infected cells, inversely correlates with cell viability, suggesting its role in COVID-19 progression and severity.

More Related Videos

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

Related Experiment Videos

Last Updated: Aug 24, 2025

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

Area of Science:

Molecular Biology
Virology
Genetics

Background:

microRNAs (miRs) are implicated as crucial molecular targets in SARS-CoV-2 infection.
Previous in silico studies identified conserved miR-like sequences in SARS-CoV-2, including miR-1307-3p, with potential roles in COVID-19.

Purpose of the Study:

To investigate the role of miR-1307-3p in SARS-CoV-2 infected cells.
To analyze the impact of miR-1307-3p expression on cell viability and associated pathways.
To explore the conservation of miR-1307-3p across SARS-CoV-2 variants.

Main Methods:

SARS-CoV-2 infection of Vero cells followed by qRT-PCR for miR expression profiling.
Transient inhibition of miR-1307-3p expression.
MTT assay for cell viability assessment.
In silico analysis of miRNOME and pathway analysis.
Small RNA sequencing to evaluate miRNOME alterations.

Main Results:

miR-1307-3p was significantly upregulated in SARS-CoV-2 infected Vero cells and inversely correlated with cell viability.
Targeted inhibition of miR-1307-3p rescued cell viability in infected cells.
In silico analysis indicated miR-1307-3p is conserved in most SARS-CoV-2 strains but absent in the BA.2 variant.
Identification of other differentially expressed miRs and associated lung disease-related genes.

Conclusions:

miR-1307-3p plays a critical role in SARS-CoV-2 infection, influencing disease progression and severity.
The absence of miR-1307-3p in the BA.2 variant may contribute to its potentially lower disease severity.
Further investigation into miR-1307-3p as a therapeutic target for COVID-19 is warranted.