Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

11.7K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.7K
Membrane Domains01:18

Membrane Domains

5.6K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.6K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.5K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.5K
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

83
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
83
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.8K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.7K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calculation of Green Function for the Problem of Mechanical Indentation of Supported Lipid Bilayer.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Marine Antimicrobial Peptide as a Promising Alternative to Polymyxin B.

Marine drugs·2026
Same author

Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions.

Bioelectrochemistry (Amsterdam, Netherlands)·2025
Same author

Quantifying the Activation Barrier for Phospholipid Monolayer Fusion Governing Lipid Droplet Coalescence.

International journal of molecular sciences·2025
Same author

Adsorption of Bovine Serum Albumin to Lipid Membranes Increases the Number and Stability of Ion Channels of Gramicidin A.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Dialectics of Antimicrobial Peptides I: Common Mechanisms of Offensive and Protecting Roles of Peptides.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Aug 24, 2025

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

16.3K

Lysolipids regulate raft size distribution.

Vladimir D Krasnobaev1,2, Timur R Galimzyanov1, Sergey A Akimov1

  • 1Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, Russia.

Frontiers in Molecular Biosciences
|October 24, 2022
PubMed
Summary
This summary is machine-generated.

Lysolipids significantly alter membrane raft domain size, influencing cellular processes and neurodegenerative disease development. This study reveals how lysolipid properties and cholesterol content modulate these critical membrane structures.

Keywords:
Alzheimer diseaseatomic force microscopycholesterolline-active componentslipid rafts∗lysolipidsneurodegenerative diseasestheory of elasticity

More Related Videos

Lipid Exchange Assay in Living Cells
08:59

Lipid Exchange Assay in Living Cells

Published on: March 21, 2025

605
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
06:32

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

2.3K

Related Experiment Videos

Last Updated: Aug 24, 2025

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

16.3K
Lipid Exchange Assay in Living Cells
08:59

Lipid Exchange Assay in Living Cells

Published on: March 21, 2025

605
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
06:32

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

2.3K

Area of Science:

  • Membrane biophysics
  • Cellular biology
  • Neuroscience

Background:

  • Cellular membranes contain lipid rafts, ordered domains crucial for signaling and protein sorting.
  • Rafts are implicated in neurodegenerative diseases, with altered lipid composition, including increased lysolipids, observed in disease states.
  • Lysolipids' impact on raft physicochemical properties and raft-involving processes is not fully understood.

Purpose of the Study:

  • To investigate the influence of lysophospholipids on liquid-ordered domains in model ternary membranes.
  • To elucidate the mechanism by which lysolipids regulate raft domain size.
  • To expand existing theoretical models of lipid-mediated domain regulation.

Main Methods:

  • Atomic force microscopy (AFM) was employed to visualize and analyze domain structures in model membranes.
  • Model ternary membranes with varying lysolipid concentrations, hydrocarbon tail saturation, and cholesterol content were utilized.
  • A theoretical model was adapted to incorporate lysolipids and dioleoylglycerol.

Main Results:

  • Even small amounts of lysolipids significantly affected membrane domain size.
  • The impact of lysolipids was dependent on the saturation of their hydrocarbon tails and the concentration of cholesterol.
  • Membrane mixtures with higher cholesterol fractions showed greater susceptibility to lysolipid-induced domain alterations.

Conclusions:

  • Lysolipids are potent regulators of membrane liquid-ordered domain size.
  • Cholesterol content modulates the sensitivity of membrane domains to lysolipids.
  • Findings contribute to understanding lipid-mediated regulation of membrane domains and their role in cellular functions and diseases.