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5-Aminolevulinic Acid-Mediated Photodynamic Therapy Improves Vocal Fold Wound Healing in Rats
Chi Zhang1,2, Wenting Pang1, Ting Gong2
1From the Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.
The Laryngoscope
|October 24, 2022
Summary
Photodynamic therapy (PDT) enhances vocal fold healing and reduces scarring in rats, both immediately after injury and during later remodeling. This minimally invasive treatment shows promise for laryngeal lesions and vocal injuries.
Area of Science:
- Otolaryngology
- Biomedical Engineering
- Regenerative Medicine
Background:
- Vocal fold scarring can impair voice quality after laryngeal injury.
- Photodynamic therapy (PDT) has shown potential in preventing scar formation in laryngeal lesions.
Purpose of the Study:
- To investigate the efficacy of PDT in improving vocal fold wound healing and reducing scar formation.
- To evaluate PDT's effects in both prophylactic and remodeling procedures in vivo.
Main Methods:
- Vocal fold stripping was performed in Sprague-Dawley rats.
- PDT was administered using 5-Aminolevulinic Acid (5-ALA) and 635 nm laser irradiation at varying doses (20, 40, 60 J/cm²).
- Prophylactic PDT was applied immediately post-surgery; remodeling PDT was applied 4 weeks post-surgery. Gene expression and histological analyses were conducted.
Main Results:
- PDT improved vocal fold wound healing in both prophylactic and remodeling settings.
- Significant increases in MMP8, MMP13, HAS2, and TGFβ1 gene expression were observed.
- Histological analysis showed increased lamina propria thickness, decreased collagen density, and increased hyaluronic acid deposition, with optimal results at 60 J/cm².
Conclusions:
- PDT effectively enhances vocal fold wound healing and reduces scarring through prophylactic and remodeling mechanisms.
- PDT represents a promising, minimally invasive treatment option for laryngeal lesions and vocal injuries to mitigate scarring.
