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Published on: November 20, 2018
Nitric oxide-mediated DNA methylation enhances cold resistance in postharvest peach fruit
Xiaoshan Guo1, Dandan Huang2, Guangqin Jing2
1College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China; Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, Xinjiang 832000, China.
Abstract:
Low temperature can affect DNA methylation. Since exogenous use of NO can reduce cold damage in peach fruit during cold storage, this study investigated the roles of NO on DNA methylation of peaches suffering cold stress. The results showed that exogenous NO effectively alleviated the decrease in total DNA methyltransferase (DNMT) activity and transcript levels induced by cold stress, whereas c-PTIO exacerbated the decrease in total DNMT activity and transcript levels. Further BSP analysis of the promoter regions of four cold resistance genes (PpCBF5-IS2, PpICE1-IS, PpMYC2-IS, PpCOR-IS1) in peaches showed that in peaches treated with exogenous NO, PpCBF5-IS2 and PpICE1-IS were modified by hypermethylation, PpMYC2-IS was modified by methylation, PpCOR-IS1 was modified by demethylation and insensitive to NO. It was suggested that NO could enhance the cold resistance of postharvest peaches by mediating DNA methylation.
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