Handling Diapause Embryos of the African Turquoise Killifish Nothobranchius furzeri

The African turquoise killifish Nothobranchius furzeri is an emerging research organism known for its short life span and long-term diapause. Diapause is a unique dormant state that suspends embryonic development without tradeoffs in the adulthood life span. Recently, diapause has been gaining increasing interest from the research community. Here, we report our methods for handling the embryos of N. furzeri that go through diapause. We focus on a few key steps: (1) collecting N. furzeri embryos, (2) sorting embryos entering diapause, (3) storing diapause embryos, (4) screening embryos exiting diapause, and (5) hatching post-diapause and fully developed embryos. This method should help the need to obtain a large number of embryos in synchronization with their diapause-entering and -exiting status and satisfy the need for cell biology, genetic, genomic, and biochemistry experiments.