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An orthogonal dual-regulation strategy for sensitive biosensing applications
Xian Yang1,2, Jinhua Wang1,3, Zhongfeng Gao1,4
1State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
National Science Review
|October 26, 2022
Summary
This study introduces a novel dual-regulation strategy for precise droplet motion control in biosensing. This innovation enhances sensitivity and allows adjustable dynamic ranges for detecting various biomolecules.
Area of Science:
- Biotechnology
- Materials Science
- Analytical Chemistry
Background:
- Droplet-based biosensing systems are crucial but limited by sensitivity and dynamic range issues.
- Precise manipulation of droplet motion on surfaces remains a significant challenge in biosensor development.
Purpose of the Study:
- To develop an orthogonal dual-regulation strategy for precise droplet motion control.
- To demonstrate a sensitive biosensing system with controllable dynamic ranges for various bio-targets.
Main Methods:
- Utilizing a lubricant-infused structural surface with micro-grooves for droplet motion control.
- Independently adjusting liquid-phase resistance (via DNA chain length) and solid-phase resistance (via sliding direction).
- Confirming the orthogonality of hydrophobic interaction and micro-groove resistance.
Main Results:
- Achieved precise control over bio-droplet motion behaviors.
- Demonstrated sensitive detection of adenosine triphosphate, miRNA, thrombin, kanamycin, and discrimination of five DNA types.
- Showcased controllable dynamic ranges for biosensing applications.
Conclusions:
- The orthogonal dual-regulation strategy offers precise control for droplet motion in biosensing.
- This approach enables sensitive detection with adjustable dynamic ranges for diverse bio-targets.
- The strategy provides significant insights for super-wettable biosensors and visual inspection technologies.
