Related Experiment Video
Updated: Aug 24, 2025
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
[Optical properties of the cornea after different modifications of penetrating keratoplasty]
E A Budnikova1, S V Trufanov1, I A Novikov1
1Research Institute of Eye Diseases, Moscow, Russia.
Purpose:
Comparative analysis of optical properties of the cornea after different modifications of penetrating keratoplasty.
Material And Methods:
The study included 56 patients (56 eyes) divided into three study groups according to the surgery technique. Group I included 17 patients (17 eyes) after one-piece mushroom keratoplasty, group II - 21 patients (21 eyes) after two-piece mushroom keratoplasty and group III - 18 patients (18 eyes) after penetrating keratoplasty. In addition to standard examination methods, before surgery and in the course of the follow-up all patients underwent software-powered corneal densitometry on the anterior segment analyzer (Pentacam HR, "Oculus", Germany), as well as an assessment of the degree and regularity of postoperative astigmatism.
Results:
Comparison of the obtained results revealed with a high degree of confidence higher values of the total specific light scattering after two-piece mushroom keratoplasty throughout the entire observation period (p<0.05). At the same time, there was no statistically significant difference in visual acuity between the three study groups (p>0.05). A statistically significant increase in the level of induced astigmatism was revealed after penetrating keratoplasty.
Conclusion:
The observed increase in light scattering after two-piece mushroom keratoplasty does not lead to a decrease in visual acuity. The interface in the optical zone of the cornea formed by a microkeratome does not affect the degree and regularity of induced astigmatism.
