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Related Experiment Video

Updated: Aug 24, 2025

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Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI.

Han Guo1, Bowen Deng1, Luming Zhao1

  • 1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, China.

Toxins
|October 26, 2022
PubMed
Summary

Researchers developed a novel aptamer, MBMI-01c, for detecting the highly toxic α-conotoxin MI (CTX-MI). This aptamer enables rapid and specific CTX-MI detection, offering a potential tool for managing this dangerous toxin.

Keywords:
alpha-conotoxin MI (CTX-MI)aptameraptasensorcomputer simulationhigh-throughput sequencing (HTS)magnetic beads SELEX (MB-SELEX)

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • α-conotoxin MI (CTX-MI) is a potent neurotoxin posing significant health risks due to its toxicity and lack of specific detection methods.
  • The absence of specific recognition elements hinders the development of effective countermeasures and diagnostic tools for CTX-MI.
  • Aptamers offer a promising alternative to traditional recognition elements, with demonstrated utility in molecular detection and drug development.

Purpose of the Study:

  • To screen and characterize aptamers with high affinity and specificity for α-conotoxin MI (CTX-MI).
  • To develop a sensitive and rapid aptasensor for CTX-MI detection.
  • To establish a versatile aptamer screening methodology applicable to other conotoxins.

Main Methods:

  • Programmed screening process to identify CTX-MI specific aptamers.
  • Characterization of aptamer affinity using affinity constant (KD) measurements.
  • Development of a biolayer interferometry (BLI) based aptasensor.
  • Evaluation of aptasensor performance including precision, specificity, and limit of detection (LOD).

Main Results:

  • An aptamer, MBMI-01c, was isolated with a high affinity (KD = 0.524 μM) for CTX-MI.
  • MBMI-01c forms an antiparallel G-quadruplet (GQ) structure crucial for specific CTX-MI recognition.
  • The developed BLI-based aptasensor demonstrated high precision, specificity, and repeatability with an LOD of 0.26 μM.
  • The aptasensor achieved rapid CTX-MI detection within 10 minutes.

Conclusions:

  • MBMI-01c is a highly effective aptamer for specific CTX-MI recognition.
  • The developed aptasensor offers a precise, rapid, and repeatable method for CTX-MI detection.
  • The aptamer holds potential for CTX-MI enrichment, detoxification, and biological studies.
  • The aptamer screening methodology is adaptable for other conotoxins, expanding its utility.