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Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Related Experiment Video

Updated: Aug 24, 2025

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Study on Tissue Homogenization Buffer Composition for Brain Mass Spectrometry-Based Proteomics.

Adam Aleksander Karpiński^1,2, Julio Cesar Torres Elguera³, Anne Sanner⁴

¹Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

|October 27, 2022

Summary

This summary is machine-generated.

Choosing the right homogenization buffer is key for brain tissue proteomics. Detergent-based buffers (DB) are most effective for extracting diverse proteins, including those crucial for neurodegenerative disorder research.

Keywords:

brain proteomics proteomic analysis sample preparation

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Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

Area of Science:

Proteomics
Neuroscience
Biochemistry

Background:

Mass spectrometry-based proteomics requires meticulous sample preparation for accurate proteome analysis.
Understanding protein extraction efficiency is vital for reliable proteomic results, especially in complex tissues like the brain.

Purpose of the Study:

To evaluate the impact of different homogenization buffer compositions on protein extraction yield and profile from brain tissue.
To determine the optimal buffer for comprehensive proteomic analysis of brain tissue, focusing on key protein classes.

Main Methods:

Comparison of three homogenization buffers: detergent-based buffer (DB), chaotropic agent-based buffer (CAB), and a detergent- and chaotropic agent-free buffer (DFB).
Label-free quantitative protein analysis using mass spectrometry to assess protein extraction efficiency and identify extracted proteins.

Main Results:

Detergent-based buffer (DB) demonstrated superior performance in protein extraction from brain tissue compared to CAB and DFB.
DB facilitated the most efficient extraction of membrane proteins, synaptic and synaptic membrane proteins, ribosomal, mitochondrial, and myelin sheath proteins.

Conclusions:

The composition of the homogenization buffer significantly influences the outcome of brain tissue proteomic analysis.
Detergent-based buffers are recommended for global proteomic profiling of brain tissue, particularly for studies involving neurodegenerative disorders due to enhanced extraction of relevant protein types.