Glucose Incorporated Graphite Matrix for Electroanalysis of Trimethoprim

Area of Science:

• Electrochemistry
• Analytical Chemistry
• Environmental Science

Background:

• Trimethoprim (TMP) is an antibiotic frequently used with sulfonamides.
• Excretion of active TMP poses risks to human health and the environment.
• A need exists for sensitive, rapid, and cost-effective analytical methods to monitor TMP.

Purpose of the Study:

• To develop and validate a novel electrochemical method for TMP analysis.
• To utilize a glucose-modified carbon paste electrode (G-CPE) for TMP detection.
• To assess the method's applicability in clinical, biological, and environmental samples.

Main Methods:

• Electrochemical analysis of TMP using a G-CPE.
• Voltammetric techniques, including differential pulse voltammetry (DPV).
• Optimization of parameters like pH and scan rate; investigation of electrochemical behavior and reaction mechanisms.

Main Results:

• Established optimal conditions for TMP detection (pH 3.4, 0.2 M PBS).
• Determined that proton transfer and diffusion phenomena govern TMP electro-oxidation.
• Achieved quantitative estimation of TMP in the range of 9.0 × 10^-7 to 1.0 × 10^-4 M.
• Demonstrated successful analysis in tablet, spiked water, and urine samples.

Conclusions:

• The developed G-CPE and voltammetric method provide a rapid, simple, sensitive, and cost-effective approach for TMP monitoring.
• The method is suitable for analyzing TMP in diverse real-world samples.
• This contributes to better management of TMP in clinical and environmental contexts.