Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tau T205 phosphorylation modulates engram cell recruitment and remote memory in mice.

Nature communications·2026
Same author

Flash Assembloids: A Rapid Biofabrication of a Platform for Modeling Early Glioblastoma Invasion at the Glioblastoma-Brain Organoid Interfaces.

Advanced healthcare materials·2026
Same author

Development of a targeted BioPROTAC degrader selective for misfolded SOD1.

Nature communications·2025
Same author

The emerging roles of long non-coding RNAs in the nervous system.

Nature reviews. Neuroscience·2025
Same author

Cross-tissue immune profiling of APOE ε4 reveals early dysregulation in Alzheimer's disease.

Research square·2025
Same author

Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes.

Frontiers in genome editing·2025

Related Experiment Video

Updated: Aug 23, 2025

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.2K

Efficient Gene Expression in Human Stem Cell Derived-Cortical Organoids Using Adeno Associated Virus.

Ann-Na Cho1, Fiona Bright1, Nicolle Morey1

  • 1Dementia Research Centre, Macquarie Medical School, Faculty of Medicine Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia.

Cells
|October 27, 2022
PubMed
Summary

Adeno-associated viruses (AAVs) offer an easier method for gene expression in cortical organoids, enabling rapid creation of disease models for neurological conditions like ALS and FTD.

Keywords:
Adeno-associated viruses (AAVs)TDP-43cortical organoidsgene deliveryneurodegenerative disease

More Related Videos

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

3.2K
Intracranial Injection of Adeno-associated Viral Vectors
08:47

Intracranial Injection of Adeno-associated Viral Vectors

Published on: November 17, 2010

47.8K

Related Experiment Videos

Last Updated: Aug 23, 2025

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.2K
Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

3.2K
Intracranial Injection of Adeno-associated Viral Vectors
08:47

Intracranial Injection of Adeno-associated Viral Vectors

Published on: November 17, 2010

47.8K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Gene Therapy

Background:

  • Cortical organoids, derived from pluripotent stem cells, offer advanced recapitulation of human brain development in vitro.
  • Adeno-associated viruses (AAVs) are increasingly utilized for central nervous system (CNS) gene delivery and therapy.

Purpose of the Study:

  • To compare adeno-associated viruses (AAVs) with traditional methods for gene expression in cortical organoids.
  • To evaluate the efficiency of AAVs in generating rapid disease models using TDP-43 variants.

Main Methods:

  • Gene expression analysis in cortical organoids using AAVs.
  • Comparison of AAVs against lipofectamine and electroporation for gene delivery.
  • Generation of disease models by expressing TDP-43 variants in organoids.

Main Results:

  • AAVs demonstrate ease-of-use for gene expression in cortical organoids.
  • AAV-mediated gene delivery facilitates rapid generation of disease models.
  • The study successfully modeled diseases linked to TDP-43 dysfunction.

Conclusions:

  • AAVs represent a powerful and efficient tool for gene manipulation in cortical organoids.
  • This approach accelerates the development of in vitro models for studying neurodegenerative diseases.
  • AAVs provide a versatile platform for CNS gene therapy research using organoid models.