Related Experiment Video
Updated: Aug 23, 2025
12:13
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
Published on: November 19, 2019
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Androgen-Responsive Oncogenic lncRNA RP11-1023L17.1 Enhances c-Myc Protein Stability in Prostate Cancer
Wenhua Huang1, Qin Chen1, Yali Lu1
1State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Science, Fudan University, Shanghai 200433, China.
International Journal of Molecular Sciences
|October 27, 2022
Summary
Long noncoding RNA RP11-1023L17.1 acts as an oncogene in prostate cancer (PCa) by stabilizing c-Myc. Its depletion inhibits PCa cell growth and promotes apoptosis, revealing new therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Prostate cancer (PCa) pathophysiology involves androgen signaling.
- The role of androgen-responsive long noncoding RNAs (lncRNAs) in PCa is not well understood.
- lncRNAs are increasingly recognized as key players in cancer development.
Purpose of the Study:
- To investigate the function of the highly expressed lncRNA RP11-1023L17.1 in PCa.
- To elucidate the mechanism by which RP11-1023L17.1 contributes to PCa tumorigenesis.
Main Methods:
- Androgen receptor manipulation in PCa cells.
- lncRNA expression analysis (microarray).
- Cell proliferation, migration, apoptosis, and cell cycle assays.
- Western blotting and mRNA expression analysis.
Main Results:
- RP11-1023L17.1 is upregulated in PCa and repressed by the androgen receptor.
- Depletion of RP11-1023L17.1 inhibits PCa cell proliferation, migration, and cell cycle, while promoting apoptosis.
- RP11-1023L17.1 promotes PCa tumorigenesis by enhancing c-Myc protein stability via repression of FBXO32.
- RP11-1023L17.1 depletion downregulates the c-Myc transcription signature.
Conclusions:
- RP11-1023L17.1 functions as an oncogene in PCa.
- RP11-1023L17.1 promotes PCa progression through a mechanism involving c-Myc stabilization.
- Targeting RP11-1023L17.1 may offer a novel therapeutic strategy for PCa.
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