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Transcriptomic-Metabolomic Profiling in Mouse Lung Tissues Reveals Sex- and Strain-Based Differences
Jolyn Fernandes1, Katelyn Dunigan-Russell2, Hua Zhong1
1Section of Neonatal-Perinatal Medicine, Department of Pediatrics, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Metabolites
|October 27, 2022
Summary
Murine strain significantly impacts lung antioxidant pathways more than sex. These strain-dependent differences in antioxidant systems may explain variations in preclinical mouse models and affect clinical translation.
Area of Science:
- Pulmonary toxicology
- Systems biology
- Genomics and metabolomics
Background:
- Omics analyses are crucial for understanding physiological and pathological processes.
- Sex is a recognized variable in pulmonary oxidant exposure studies, but murine strain effects are often overlooked.
Purpose of the Study:
- To investigate sex- and strain-dependent pathways in adult mouse lungs using integrated multi-omics.
- To determine the relative influence of sex versus murine strain on lung responses.
Main Methods:
- High-resolution metabolomic profiling and RNA-sequencing were employed in an unbiased manner.
- Integrated analysis of metabolomic and transcriptomic data to identify sex- and strain-dependent pathways.
Main Results:
- Murine strain had a greater impact than sex on inflammatory and oxidant/antioxidant response pathways.
- Differences in glutathione and selenium metabolism pathways were observed between strains.
- Transcriptomic analysis showed strain-dependent variations in key antioxidant and inflammatory genes.
Conclusions:
- Murine strain significantly influences baseline expression of lung antioxidant systems.
- Strain-specific differences in preclinical models may lead to variability and impact clinical translation of findings.
