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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Related Experiment Video

Updated: Aug 23, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

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Developments in FRET- and BRET-Based Biosensors.

Yuexin Wu1, Tianyu Jiang2,3

  • 1School of Life Sciences, Peking University, Beijing 100871, China.

Micromachines
|October 27, 2022
PubMed
Summary
This summary is machine-generated.

Fluorescence and bioluminescence resonance energy transfer biosensors are powerful tools for molecular sensing and biological process monitoring. This review covers recent advancements and applications in biomedicine, environmental science, and synthetic biology.

Keywords:
bioluminescence resonance energy transfer (BRET)biosensorsfluorescence resonance energy transfer (FRET)imagingimmunosensorsnanosensorswhole-cell sensors

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Resonance energy transfer (RET) technologies, including fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET), are pivotal in analytical sciences.
  • These RET techniques offer versatile strategies for developing sophisticated biosensors.

Purpose of the Study:

  • To provide a comprehensive overview of recent advancements in FRET- and BRET-based biosensors.
  • To highlight the diverse applications of these biosensors in biomedicine, environmental monitoring, and synthetic biology.
  • To discuss design strategies, current limitations, and future prospects for RET-based biosensor development.

Main Methods:

  • Review of recent scientific literature on FRET- and BRET-based biosensors.
  • Analysis of design principles and application examples.
  • Discussion of limitations and future research directions.

Main Results:

  • FRET and BRET are effectively employed in designing biosensors for various analytical and monitoring tasks.
  • These biosensors have demonstrated significant utility across biomedicine, environmental science, and synthetic biology.
  • Various design strategies have been successfully implemented, showcasing the adaptability of FRET and BRET.

Conclusions:

  • FRET- and BRET-based biosensors represent a significant technological advancement with broad applicability.
  • Continued development in design and application is expected to further enhance their impact in scientific research and practical uses.
  • Addressing current limitations will pave the way for expanded future applications of these powerful sensing tools.