[Statistical analysis of results in the determination of steroid hormone receptors]

Statistical evaluation of the data of steroid hormone receptors estimation involved the principle of dependence of each experimentally obtained magnitude of total binding (in presence of a single labelled ligand) on the magnitude of unspecific binding (at the similar content of labelled ligand but in addition of unlabelled substance excess). The principle enabled to calculate: individual values of specific binding as a difference between each value for total and unspecific binding independently on amount of parallel probes of total and unspecific bindings, mean value of specific binding using the calculated magnitudes, standard deviation (dispersion) and standard error of mean value. Statistical method allowed to determine differences between magnitudes of specific binding (content of receptors) at saturating concentrations of ligand as well as to estimate standard deviation of KD and Vmax in a plot. The statistical principles were used for estimation of specific binding of 3H-estra dil-17 beta, 3H-dihydrotestosterone and 3H-5 alpha-and-rostan-3 beta, 17 beta-diol in various rat tissues (hypophysis, prostate, uterus) and human tissues (mammary gland tissue tumor, prostate adenoma). The principle developed may be used for estimation of various receptors of biologically active substances, where the specific binding is calculated as a difference between total and unspecific binding.