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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Published on: July 4, 2011
Stimuli-responsive fluorescent nanogel: a nonconventional donor for ratiometric temperature and pH sensing
Soumen Ghosh1, Mohd Avais1, Subrata Chattopadhyay1
1Department of Chemistry, Indian Institute of Technology Patna, Bihta, Patna, 801106, India. sch@iitp.ac.in.
Abstract:
A reactive stimuli responsive fluorescent polyaminoamide nanogel (NANO-PAMAM) is synthesized via an aza-Michael polyaddition reaction in water. In the next stage, doxorubicin (a FRET acceptor) is covalently linked with the nanogel (NANO-PAMAM, a nonconventional FRET donor) to form a ratiometric nanosensor for temperature and pH sensing. MTT assay revealed comparable biocompatibility of the donor nanogels (NANO-PAMAM) and nanosensors (NANO-PAMAM-DOX).

