Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An analytic, moment-based method to estimate orthopositronium lifetimes in positron annihilation lifetime spectroscopy measurements.

Bio-algorithms and med-systems·2026
Same author

A Novel Approach to In Vivo EPR Spectroscopy for Repeatable Assessments of Oxygenation Levels in Tumors at Any Depth: Preliminary Feasibility Studies Utilizing a Multisite Oxygen Sensor Inside HDR Brachytherapy Needles.

Advances in experimental medicine and biology·2026
Same author

Letting Biology Validate the EPR Imaging of Tumor pO<sub>2</sub>.

Advances in experimental medicine and biology·2026
Same author

Trityl OXO71 Pharmacokinetics and Distribution in Rhesus Macaques.

Magnetic resonance in medicine·2025
Same author

Sensitivity Enhancement in Pulsed Hyperfine EPR Spectroscopy with Hadamard-Encoded Acquisition.

The journal of physical chemistry letters·2025
Same author

A novel high order directional total variation algorithm of EPR imaging for fast scan.

Journal of X-ray science and technology·2025
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Aug 23, 2025

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.5K

An iterative reconstruction algorithm without system matrix for EPR imaging.

Zhiwei Qiao1, Yang Lu1, Peng Liu2

  • 1School of Computer and Information Technology, Shanxi University, Taiyuan, Shanxi 030006, China.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|October 29, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel iterative reconstruction algorithm for electron paramagnetic resonance (EPR) imaging that eliminates the need for a system matrix. This R-TVcDM method simplifies calculations and reduces memory requirements for accurate oxygen imaging.

Keywords:
EPR imagingImage rotationIterative algorithmOptimizationSystem matrix

More Related Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

6.9K
Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance
07:07

Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance

Published on: February 14, 2025

2.8K

Related Experiment Videos

Last Updated: Aug 23, 2025

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.5K
Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

6.9K
Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance
07:07

Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance

Published on: February 14, 2025

2.8K

Area of Science:

  • Medical Imaging
  • Biophysics
  • Computational Imaging

Background:

  • Electron paramagnetic resonance (EPR) imaging is a key oxygen imaging technique for image-guided radiation therapy.
  • Iterative reconstruction algorithms are crucial for EPR imaging, but often require complex system matrices that are computationally intensive and memory-demanding.
  • Existing methods rely on rotating scanners and stationary objects, posing computational challenges.

Purpose of the Study:

  • To develop a simplified iterative reconstruction algorithm for EPR imaging that avoids system matrix computation and storage.
  • To enhance the efficiency and accessibility of EPR imaging reconstruction.
  • To explore alternative projection and backprojection strategies for improved imaging.

Main Methods:

  • Proposed an iterative reconstruction algorithm without a system matrix for EPR imaging, named R-TVcDM (image-rotation based TVcDM).
  • Implemented projection and backprojection operations by rotating the object while keeping the scanning device stationary.
  • Utilized a commonly-used image-rotation algorithm as the core computational component, bypassing system matrix calculations.

Main Results:

  • The R-TVcDM algorithm achieved comparable reconstruction accuracy to the original TVcDM algorithm using real projection data.
  • Successfully avoided the complex calculation and substantial memory storage associated with the system matrix.
  • Demonstrated a significant simplification of the iterative reconstruction process for EPR imaging.

Conclusions:

  • The R-TVcDM algorithm offers a computationally efficient and memory-saving alternative for EPR imaging reconstruction.
  • The developed method maintains high reconstruction accuracy, making advanced oxygen imaging more feasible.
  • The principles of object rotation for projection/backprojection may be applicable to other imaging modalities like CT and PET.