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Published on: October 8, 2014
14 C-paraquat Efflux Assay in Arabidopsis Mesophyll Protoplasts
Jin-Qiu Xia1, Qian-Qian Liu1, Cheng-Bin Xiang1
1Division of Life Sciences and Medicine, Division of Molecular & Cell Biophysics, Hefei National Science Center for Physical Sciences at the Microscale, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science and Technology of China, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Hefei, Anhui Province 230027, China.
Abstract:
Weeds compete with crops for growth resources, causing tremendous yield losses. Paraquat is one of the three most common non-selective herbicides. To study the mechanisms of paraquat resistance, we need to trace the movement of paraquat in plants and within the cell. 14 C is a radioactive carbon isotope widely used to trace substances of interest in various biological studies, especially in transport analyses. Here, we describe a detailed protocol using 14 C-paraquat to demonstrate paraquat efflux in Arabidopsis protoplasts.
