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Updated: Aug 23, 2025

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Digital Protein Detection in Bulk Solutions.

Sungjun Beck1, Donghae Shin1, Sun Jin Kim2

  • 1Department of Biological Chemistry, University of California, Irvine, Irvine, California 92697, United States.

ACS Omega
|October 31, 2022
PubMed
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This summary is machine-generated.

A novel 3D particle counter offers sensitive and rapid protein detection for medical diagnostics. This technology simplifies assays, bridging the gap between high sensitivity and quick results for disease diagnosis.

Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Assay Development

Background:

  • Clinical laboratory diagnostics often involve specialized equipment with trade-offs between sensitivity, specificity, and ease of use.
  • Existing methods like chromatography-mass spectrometry offer high analytical sensitivity but require complex operation, while simple assays lack sensitivity.

Purpose of the Study:

  • To present an application study of 3D particle counter technology for protein detection.
  • To address the limitations of current diagnostic tools by improving analytical sensitivity and assay turnover time.
  • To simplify protein detection through bead-based immunoassays.

Main Methods:

  • Utilized 3D particle counter technology based on optical confocal detection to scan large sample volumes (0.5-3 mL).

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  • Employed bead-based immunoassays for protein detection.
  • Assessed the detection of recombinant mouse interleukin 6.

    • Main Results:

    • Achieved a subpicomolar limit of detection, ranging from 119 to 346 fM.
    • Completed the assay within a 3.5-hour timeframe.
    • Demonstrated the 3D particle counter's capability as an accessible and flexible alternative to flow cytometry.

    Conclusions:

    • The 3D particle counter technology, combined with bead-based immunoassays, effectively bridges the gap between analytical sensitivity and assay time.
    • This approach offers a simplified and accessible method for sensitive protein detection in medical diagnostics.
    • The technology presents a viable alternative to existing methods, enhancing diagnostic capabilities.