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LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology
Katiane Roversi1,2,3, Hamid Ebrahimi Orimi2,4, Mahyar Erfanian2,5
1Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC, Canada.
Bio-Protocol
|October 31, 2022
Summary
Laser Induced Side Transfer (LIST) is a novel bioprinting method for creating tissue-like structures. This technology enables the precise printing of delicate cells, like neurons, offering a promising alternative to animal testing and organ transplantation.
Area of Science:
- Biotechnology
- Regenerative Medicine
- Biofabrication
Background:
- Cell bioprinting fabricates tissue-like constructs for drug development and organ transplantation.
- Existing methods face challenges with delicate cell types.
Purpose of the Study:
- Introduce and detail the Laser Induced Side Transfer (LIST) bioprinting technology.
- Provide a reproducible protocol for bioprinting delicate cell types, specifically primary neurons.
Main Methods:
- Laser-assisted drop-on-demand bioprinting using the LIST technique.
- Detailed protocol covering cell harvesting, bio-ink preparation, laser setup, printing, and analysis.
- Focus on a unique laser setup adaptable for biology labs.
Main Results:
- Demonstrated successful bioprinting of delicate cell types, including primary neurons, using the LIST technology.
- Provided a comprehensive protocol facilitating reproduction of the bioprinting process.
Conclusions:
- The LIST technology offers a versatile platform for bioprinting various cell types.
- This protocol can advance research in tissue engineering, drug testing, and regenerative medicine.
Keywords:
3D bio-printingDrop-on-demandLaser-assisted bioprintingLaser-induced side transferNociceptor neuronsTissue engineering
