Related Experiment Video

Updated: Jul 20, 2026

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
08:37

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

2.3K

Molecular analysis of oxa-48 producing k. pneumoniae strains isolated from patients with catheter-associated sepsis

Ö Akgül1, G Bora

  • 1Department of Pharmaceutical Microbiology, Health Sciences Institute, Faculty of Pharmacy, Van Yüzüncü Yıl University, Van, Turkey. o.akgul@yyu.edu.tr.

European Review for Medical and Pharmacological Sciences
|October 31, 2022
PubMed
Abstract

Insights

Multidrug-resistant Klebsiella pneumoniae strains, particularly carbapenem-resistant ones, pose a significant public health threat. This study identified 24 such strains from intensive care units, all belonging to the ST11 sequence type.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Klebsiella pneumoniae is a major cause of hospital and community-acquired infections.
  • Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains are a growing global public health concern.
  • Understanding the genetic relatedness and resistance profiles of CRKP is crucial for effective infection control.

Purpose of the Study:

  • To sequence and phylogenetically analyze Klebsiella pneumoniae strains isolated from blood cultures of intensive care unit patients.
  • To investigate the prevalence of multidrug resistance and specific resistance genes within these isolates.
  • To determine the sequence type (ST) of the identified K. pneumoniae strains.

Main Methods:

  • Isolation and identification of K. pneumoniae from blood cultures of patients with catheter-related sepsis.
  • Antimicrobial susceptibility testing, including resistance against common antibiotics.
  • Molecular analyses, including multilocus sequence typing (MLST) and detection of carbapenemase genes (e.g., OXA-48).

Main Results:

  • Out of 276 K. pneumoniae isolates, 24 multidrug-resistant strains were selected for further analysis.
  • The highest resistance rates were observed against Ampicillin, Amoxicillin/Clavulanic Acid, Ceftazidime, and Ceftriaxone.
  • Phylogenetic analysis indicated relatedness among K. pneumoniae isolates from different countries concerning the OXA-48 gene.
  • Multilocus sequence typing revealed that all 24 K. pneumoniae isolates belonged to sequence type 11 (ST11).

Conclusions:

  • The study identified a clonal ST11 Klebsiella pneumoniae population among multidrug-resistant isolates from intensive care unit patients.
  • The findings highlight the importance of ST11 as a globally significant sequence type associated with carbapenem resistance.
  • Continuous surveillance and molecular characterization of K. pneumoniae are essential for managing and controlling the spread of resistant strains.

More Related Videos

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

6.7K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.3K

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...