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A Cristae-Like Microcompartment in Desulfobacterota.

Shawn Erin McGlynn1,2,3,4, Guy Perkins5,6, Min Sub Sim7

  • 1Earth-Life Science Institute, Tokyo Institute of Technology, Meguro, Tokyo, Japan.

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Intracytoplasmic membranes (ICMs) in sulfate-reducing bacteria resemble mitochondrial cristae. These structures, found in Desulfobacterota and Desulfovibrio carbinolicus, suggest analogous, not homologous, protein systems evolved.

Keywords:
Desulfobacterotaanaerobic oxidation of methanecompartmentalizationelectron microscope tomographyintracytoplasmic membraneouter membrane vesiclesperapera junctionsulfate reduction

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Area of Science:

  • Microbial ultrastructure and evolution
  • Cell biology of prokaryotes
  • Mitochondrial origins

Background:

  • Alphaproteobacteria possess intracytoplasmic membranes (ICMs) homologous to mitochondrial cristae proteins.
  • This raises the hypothesis of pre-evolved cristae-like structures in Alphaproteobacteria endosymbionts.
  • Limited knowledge of microbial fine structure allows for possibilities beyond Alphaproteobacteria.

Purpose of the Study:

  • To characterize lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota.
  • To investigate similar structures in Desulfovibrio carbinolicus.
  • To compare these structures with mitochondrial cristae architecture.

Main Methods:

  • Detailed characterization of lamellar cristae-like ICMs using advanced electron microscopy.
  • Three-dimensional electron microscopy for nanoscale attachment analysis.
  • Comparative structural analysis with opisthokont mitochondria.

Main Results:

  • Discovery of lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota and Desulfovibrio carbinolicus.
  • ICMs are junction-bound to the cytoplasmic membrane, resembling lamellar cristae in opisthokont mitochondria.
  • Key proteins for cristae formation are absent in Desulfobacterota, indicating analogous evolution.

Conclusions:

  • The taxonomic breadth of microorganisms with ICMs is expanded.
  • Structural similarity suggests convergent evolution of cristae-like structures in Desulfobacterota.
  • Findings enhance understanding of three-dimensional microbial fine structure and evolutionary processes.