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Published on: July 6, 2019
Modeling of Chronic Lung Inflammation in Rats by Repeated Intratracheal Administration of LPS
V A Palikov1, Yu A Palikova1, P A Rudenko1
1Branch of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Abstract:
A model of a chronic lung inflammation in SPF Sprague-Dawley rats was developed by repeated intratracheal administration of LPS in a dose of 0.4 mg/kg. On day 22 of the study, male rats treated with LPS have relative monocytopenia and reduced mean concentration of hemoglobin in the erythrocyte and the mean platelet volume in comparison with the control animals (saline). Intratracheal administration of LPS induced an inflammatory process in the lungs characterized by focal atelectasis, compensatory emphysematous expansion of subpleural pulmonary acini, focal mononuclear and neutrophilic perivascular and peribronchial infiltration, and minor focal mononuclear and neutrophilic infiltration of the alveolar walls. Against the background of LPS administration, germinal centers appeared in the lymphoid follicles of the white pulp of the spleen, and focal mononuclear infiltration of the tracheal mucosa and/or submucosa was observed in some animals.
Insights
This study developed a rat model for chronic lung inflammation using lipopolysaccharide (LPS). LPS exposure caused lung damage and altered blood cell counts, including monocytopenia and reduced hemoglobin.
Area of Science:
- Pulmonary Medicine
- Toxicology
- Immunology
Background:
- Chronic lung inflammation models are crucial for understanding respiratory diseases.
- Lipopolysaccharide (LPS) is a potent inducer of inflammation.
- Developing reliable animal models is essential for preclinical research.
Purpose of the Study:
- To establish and characterize a rat model of chronic lung inflammation.
- To investigate the hematological and pathological changes induced by repeated LPS administration.
Main Methods:
- SPF Sprague-Dawley rats were used.
- Chronic lung inflammation was induced via repeated intratracheal administration of LPS (0.4 mg/kg).
- Hematological parameters and lung/spleen/trachea tissues were analyzed on day 22.
Main Results:
- LPS administration induced lung inflammation with atelectasis, emphysema, and cellular infiltration (mononuclear and neutrophilic).
- Rats exposed to LPS exhibited relative monocytopenia, reduced erythrocyte hemoglobin concentration, and decreased mean platelet volume.
- Spleen lymphoid follicles showed germinal centers, and tracheal mucosa displayed mononuclear infiltration.
Conclusions:
- Repeated intratracheal LPS administration effectively models chronic lung inflammation in rats.
- This model elicits significant pulmonary pathology and systemic hematological alterations.
- The model provides a valuable tool for studying inflammatory lung diseases and potential therapeutic interventions.
