Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phorbol ester-induced orbital myositis.

P D Fries, D S Fohrman, D H Char

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |September 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Iris ring melanoma: fine needle biopsy.

    The British journal of ophthalmology·2006
    Same author

    20 MHz high frequency ultrasound assessment of scleral and intraocular conjunctival squamous cell carcinoma.

    The British journal of ophthalmology·2002
    Same author

    Uveal tumour resection.

    The British journal of ophthalmology·2001
    Same author

    Early wound dehiscence with use of hydroxyapatite orbital implant covered with calf pericardium.

    The British journal of ophthalmology·2001
    Same author

    Objective evaluation of improvement in optic neuropathy following radiation therapy for thyroid eye disease, by S. Rush, J. M. Winterkorn, and R. Zak. Int J. Radiat Oncol Biol. Phys 47:191-4, 2000.

    Survey of ophthalmology·2001
    Same author

    Amelanotic corneal melanoma after a blast injury.

    American journal of ophthalmology·2001
    Same journal

    The economics of the initial preventive physical examination in medicare-reply.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    Modification of silicone oil retention sutures in aphakic eyes with iris loss-reply;.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    December 2011 archives web quiz winner.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    Angle involvement and glaucoma in patients with biopsy-proven iris melanoma: a response-reply.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    About this journal.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    In memoriam: goodwin m. Breinin, MD (1918-2011).

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    See all related articles

    A new rabbit model using 12-O-tetradecanoyl-phorbol-13-acetate (TPA) injection effectively mimics human orbital myositis. This model shows inflammation, fibrosis, and restricted eye muscle movement, proving useful for studying the condition.

    Area of Science:

    • Ophthalmology
    • Musculoskeletal Research
    • Pathology

    Background:

    • Idiopathic orbital myositis and thyroid orbital myopathy are debilitating conditions affecting eye movement.
    • Current models may not fully capture the complex pathology of these diseases.

    Purpose of the Study:

    • To develop and validate a novel animal model for studying orbital myositis.
    • To investigate the effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on extraocular muscle function and histology.

    Main Methods:

    • Induction of inflammation and fibrosis via single superior rectus muscle injection of TPA in New Zealand white rabbits.
    • Quantitative assessment of muscle restriction using strain gauge measurements at 24 hours and 12 weeks.
    • Clinical and histological evaluation of induced changes.

    Related Experiment Videos

    Main Results:

    • TPA injection caused significant inflammation, edema, fibrosis, and muscle restriction.
    • Muscle restriction increased by 60% at 24 hours and over 400% by 12 weeks.
    • Histological findings paralleled human idiopathic orbital myositis and thyroid orbital myopathy.

    Conclusions:

    • The TPA-induced rabbit model accurately replicates key features of human orbital myositis.
    • This model provides a valuable tool for further research into the pathogenesis and treatment of orbital myopathies.
    • The study highlights the utility of TPA in creating a reproducible model of extraocular muscle disease.