Related Experiment Video
Updated: Aug 23, 2025
08:34
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
2.5K
OVARIAN TOXICITY OF FAC CHEMOTHERAPY IN RATS AND POSSIBILITY OF ITS CORRECTION WITH PLATELET-RICH PLASMA
V A Shamrai1,2, O I Misiurko1,2, D I Grebeniuk2
1Communal non-profit enterprise "Podillian Regional Oncology Center of Vinnytsia Regional Council", Vinnytsia 21000, Ukraine.
Experimental Oncology
|November 3, 2022
Summary
Chemotherapy (5-fluorouracil, doxorubicin, cyclophosphamide) harms ovarian reserve in rats. Local platelet-rich plasma (PRP) injections improved hormonal status, suggesting a potential therapeutic approach for chemotherapy-induced ovarian damage.
Area of Science:
- Reproductive endocrinology
- Oncology supportive care
- Regenerative medicine
Background:
- Chemotherapy, including 5-fluorouracil, doxorubicin, and cyclophosphamide (FAC), can lead to ovarian toxicity.
- Premature ovarian failure is a significant concern for cancer survivors, impacting fertility and hormonal balance.
- Platelet-rich plasma (PRP) contains growth factors with potential regenerative properties.
Purpose of the Study:
- To investigate the impact of FAC chemotherapy on female rat hormonal status.
- To evaluate the efficacy of local platelet-rich plasma (PRP) administration in mitigating chemotherapy-induced ovarian damage.
- To compare the dynamics of ovarian reserve markers before and after PRP treatment.
Main Methods:
- Female Wistar rats were subjected to a 4-course FAC chemotherapy regimen with 3-week intervals.
- Ovarian reserve was assessed by measuring anti-Mullerian hormone, estradiol (E2), and follicle-stimulating hormone (FSH) levels.
- Following chemotherapy, rats received intra- and periovarian PRP injections three times at weekly intervals.
Main Results:
- FAC chemotherapy induced a progressive decline in anti-Mullerian hormone and E2 levels, alongside an increase in FSH.
- These hormonal changes indicate a significant reduction in ovarian reserve.
- Subsequent PRP administration led to an improvement in the measured hormonal parameters, suggesting restoration of ovarian function.
Conclusions:
- FAC chemotherapy causes premature ovarian failure in experimental models.
- Local intra- and periovarian administration of PRP demonstrates a positive effect on hormonal parameters of ovarian reserve.
- PRP therapy presents a promising strategy for managing chemotherapy-induced ovarian toxicity.
