Evidence that post-transcriptional changes in the expression of mitogen regulated protein accompany immortalization...

Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and to secrete mitogen regulated protein (MRP, also known as proliferin). We report here that the level at which the change in gene expression occurs during this spontaneous immortalization process is post-transcriptional, reflecting a change in either RNA processing or transcript stability. To our knowledge, this is the first report of an alteration at the post-transcriptional level of gene expression that accompanies immortalization.