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Related Experiment Video

Updated: Aug 23, 2025

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
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NaBiF4 upconversion nanoparticle-based electrochemiluminescent biosensor for E. coli O157 : H7 detection.

Danqing Liu1, Xingxing Lv1, Chaoyue Zhao1

  • 1School of Material Science and Chemical Engineering, Harbin University of Science and Technology Harbin 150040 China danqingliu76@163.com wengling79@163.com.

RSC Advances
|November 4, 2022
PubMed
Summary

A novel electrochemiluminescent (ECL) immunosensor using NaBiF4:Yb3+/Er3+ upconversion nanoparticles (UCNPs) was developed for rapid pathogen detection. This biosensor offers a sensitive and selective method for detecting E. coli O157:H7 in food safety and diagnostics.

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Area of Science:

  • Nanotechnology
  • Biosensing
  • Analytical Chemistry

Background:

  • Foodborne pathogens like E. coli O157:H7 present significant risks to public health.
  • Current diagnostic methods for pathogens often lack the required speed, sensitivity, or cost-effectiveness for point-of-care applications.

Purpose of the Study:

  • To develop a novel, rapid, and sensitive electrochemiluminescent (ECL) immunosensor for the detection of E. coli O157:H7.
  • To synthesize and characterize NaBiF4:Yb3+/Er3+ upconversion nanoparticles (UCNPs) for ECL applications.

Main Methods:

  • Synthesized NaBiF4:Yb3+/Er3+ UCNPs via a room-temperature, environmentally friendly method.
  • Constructed an ECL immunosensor by assembling Au nanoparticles and Anti-E. coli O157:H7 antibody onto UCNPs.
  • Investigated the ECL behaviors of UCNPs with K2S2O8 and evaluated the immunosensor's performance for E. coli O157:H7 detection.

Main Results:

  • The synthesized UCNPs exhibited high ECL intensity and stable cathodic signals when combined with K2S2O8.
  • The developed ECL immunosensor demonstrated high sensitivity for E. coli O157:H7 detection, with a linear range from 200 to 100,000 CFU mL-1 and a limit of detection of 138 CFU mL-1.
  • The biosensor showed excellent specificity, stability, and repeatability.

Conclusions:

  • The UCNP-based ECL immunosensor provides a highly sensitive and selective platform for the rapid detection of E. coli O157:H7.
  • This strategy holds promise for applications in food safety and preclinical diagnosis, addressing the need for efficient pathogen detection assays.