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Updated: Aug 23, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
Supercycled R-symmetry sequences for robust heteronuclear polarization transfer in solid-state NMR
Lixin Liang1, Chao Shang1, Kuizhi Chen2
1State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, 2011-Collaborative Innovation Center of Chemistry for Energy Materials, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Herein, we introduce supercycle of R-symmetry sequences (SR-sequences) and incomplete supercycle schemes of R-symmetry sequences (iSR-I- and iSR-II-sequences) to improve the robustness of PRESTO for heteronuclear polarization transfer in MAS NMR. The constructions of SR- and iSR-I/II- sequences are based on the different phase-inverted supercycles of R-symmetry sequences, and such supercycles can suppress the influence of CSA, resonance offset and RF mismatch when incorporated into the PRESTO method. Moreover, the SR- and iSR-II-sequences are more efficient in suppressing the interference of homonuclear dipolar coupling. The improved robustness of SR-, iSR-I- and iSR-II-PRESTO over the original R-PRESTO has been verified by numerical simulations and NMR experiments on NH4H2PO4 and gamma-alumina at fast MAS conditions. It is also important to note that the SR- and iSR-II-PRESTO can greatly lengthen the transverse relaxation times and lead to much higher polarization transfer efficiency compared to R-PRESTO, thanks to their superior tolerance to RF inhomogeneity and homonuclear dipolar coupling.
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