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New efficient meta-fermentation process for lactic acid production from municipal solid waste.

Miguel G Acedos1, Paz Gómez-Pérez2, Tamara Espinosa3

  • 1Biotechnology Department, AINIA, Parque Tecnológico de Valencia, Paterna, Spain.

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Summary

This study optimized meta-fermentation for lactic acid production from food waste. A bioaugmentation strategy achieved high lactic acid yields, demonstrating potential for efficient biopolymer precursor manufacturing.

Keywords:
Anaerobic DigestionBioaugmentationLactic acidMeta-fermentationOFMSWScale-up

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Environmental Science

Background:

  • The global lactic acid market is projected to reach $9.8 billion by 2025, driven by polylactic acid production.
  • Meta-fermentation offers advantages over pure cultures, including reduced contamination risk and lower costs.
  • Challenges remain in enhancing conversion efficiency, minimizing by-products, and controlling meta-fermentation.

Purpose of the Study:

  • To develop a robust meta-fermentation process for efficient lactic acid production from OFMSW.
  • To establish a stable, pre-industrial scale (1500 L) process.
  • To optimize operating conditions and implement a novel bioaugmentation strategy.

Main Methods:

  • Isolation and cultivation of lactic acid bacteria (LAB) for inoculum preparation.
  • Optimization of operating conditions: temperature (55°C), hydraulic retention time (HRT, 2 days), and pH (4.8-5.7).
  • Implementation of a bioaugmentation strategy using a LAB-rich inoculum.

Main Results:

  • Lactic acid accumulation reached 41.5 gO2/L, representing over 70% of filtered COD.
  • Lactic acid was the predominant product over volatile fatty acids under all feeding strategies.
  • The bioaugmentation strategy significantly improved lactic acid yields compared to the prototype without reinoculation.

Conclusions:

  • Optimal conditions for lactic acid production from mixed cultures include 55°C, 2-day HRT, and pH 4.8-5.7 with daily pH control.
  • Bioaugmentation enhanced lactic acid production, proving effective without reinoculation.
  • Further optimization is required to increase purity and concentration for economic feasibility.