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Updated: Aug 22, 2025

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
07:55

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

494

Circulating extracellular vesicle-containing microRNAs reveal potential pathogenesis of Alzheimer's disease

Yi Wang1, Ping Yuan2, Lu Ding3

  • 1Translational Research Center, Shanghai Yangzhi Rehabilitation Hospital Affiliated to Tongji University School of Medicine, Shanghai, China.

Frontiers in Cellular Neuroscience
|November 7, 2022
PubMed

Insights

Researchers explored microRNA profiles in extracellular vesicles (EVs) from Alzheimer's disease (AD) patients. This study reveals key circulating EV-containing miRNAs (CEmiRs) linked to AD pathogenesis, offering new therapeutic targets.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Alzheimer's disease (AD) pathogenesis remains largely unknown, impeding therapeutic and diagnostic advancements.
  • Circulating extracellular vesicles (EVs) offer a promising avenue for understanding AD.
  • Altered microRNA (miRNA) profiles in EVs from AD patients suggest their role in disease.

Purpose of the Study:

  • To investigate the global miRNA profile in serum-derived EVs from AD, mild cognitive impairment (MCI), and healthy individuals.
  • To identify differentially expressed CEmiRs (DECEmiRs) and their potential targets in AD.
  • To elucidate the role of CEmiRs in AD pathogenesis and signaling networks.

Main Methods:

  • Serum-derived EVs were isolated from AD, MCI, and healthy control groups.
  • Comprehensive miRNA profiling of serum-derived EVs was performed.
  • Bioinformatic analysis was used to identify DECEmiRs, predict targets, and analyze biological functions and interactions.

Main Results:

  • Distinct miRNA expression patterns were observed in serum-derived EVs across AD, MCI, and healthy groups, classified into 10 clusters.
  • Identification of specific DECEmiRs implicated in AD pathogenesis.
  • Elucidation of complex signaling networks regulated by CEmiRs in AD.

Conclusions:

  • Circulating EV-miRNAs represent crucial regulators in Alzheimer's disease pathogenesis.
  • The study provides insights into temporal regulation of signaling networks in AD.
  • Findings support the development of novel therapeutic strategies, including multi-target drug combinations for AD.
Keywords:
Alzheimer’s diseaseExosomemiRNAneurodegenerationserum

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