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Related Experiment Video

Updated: Aug 22, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Calcite-Assisted Localization and Kinetics (CLocK) Microscopy.

Joseph W Monaghan1, Zachary J O'Dell1, Sanjay Sridhar1

  • 1Department of Chemistry, Temple University, Philadelphia, Pennsylvania19122, United States.

The Journal of Physical Chemistry Letters
|November 7, 2022
PubMed
Summary
This summary is machine-generated.

Calcite-assisted localization and kinetics (CLocK) microscopy offers multiparameter super-resolution imaging, enabling <10 nm resolution with polarization, orientation, and kinetic information. This technique provides new mechanistic insights into dynamic processes at the nanoscale.

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Area of Science:

  • Optical Microscopy
  • Nanoscale Imaging
  • Biophysics

Background:

  • Super-resolution microscopy techniques surpass the diffraction limit for enhanced spatial resolution.
  • Encoding additional information like anisotropy and orientation into super-resolved images remains a challenge.

Purpose of the Study:

  • Introduce calcite-assisted localization and kinetics (CLocK) microscopy.
  • Enable multiparameter super-resolution imaging with polarization, orientation, and kinetic information.
  • Provide mechanistic insight into dynamic nanoscale processes.

Main Methods:

  • Integration of a rotating calcite crystal into standard optical microscope setups.
  • Utilizing the unique point spread function of CLocK images for data encoding.
  • Achieving emitter/scatterer localization with <10 nm resolution.

Main Results:

  • CLocK microscopy provides immediate polarization and orientation data.
  • Enables localization with sub-10 nm spatial resolution.
  • Captures kinetic information faster than camera integration times.

Conclusions:

  • CLocK microscopy is a cost-effective and easily integrated technique for advanced super-resolution imaging.
  • It offers a powerful new tool for studying dynamic nanoscale phenomena.
  • Facilitates mechanistic understanding of processes like nanoparticle dissolution and SERS.