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Atomic Force Microscopy

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Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

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Published on: December 12, 2013

MINSTED nanoscopy enters the Ångström localization range.

Michael Weber¹, Henrik von der Emde¹, Marcel Leutenegger¹

¹Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

Nature Biotechnology

|November 7, 2022

Summary

This summary is machine-generated.

Researchers achieved Ångström-level localization precision for fluorophores using all-optical MINimal STED (MINSTED) nanoscopy at room temperature. This breakthrough enables sub-nanometer resolution imaging of cellular structures with minimal photons.

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Area of Science:

Optical microscopy
Nanotechnology
Biophysics

Background:

Super-resolution microscopy achieves nanometer localization precision.
MINimal STimulated Emission Depletion (MINSTED) nanoscopy enhances precision by increasing donut beam power.
Current techniques are limited in achieving Ångström-level resolution.

Purpose of the Study:

To demonstrate all-optical, room temperature localization of fluorophores with Ångström precision.
To apply MINSTED nanoscopy for high-resolution imaging of biological structures.
To explore the potential of MINSTED for studying macromolecular complexes.

Main Methods:

Utilized MINimal STimulated Emission Depletion (MINSTED) nanoscopy.
Employed a blue-shifted STED beam and on/off switching for fluorophore separation.
Localized individual fluorophores bound to DNA strands.

Main Results:

Achieved localization precision of 4.7 Å with 2,000 detected photons.
Demonstrated single-digit nanometer resolution imaging of nuclear pore complexes and nuclear lamin distribution.
Estimated localization precision of 2.3 Å with 10,000 detected photons.

Conclusions:

MINSTED nanoscopy enables Ångström-level localization precision at room temperature.
This technique offers unprecedented resolution for imaging cellular components.
MINSTED is poised to advance the study of macromolecular complexes within cells.