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Updated: Aug 6, 2026
08:55
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
Does SLC17A9 (VNUT) function as a stand-alone nucleotide and/or phosphate transporter?
Jennifer Struck1, Vivek Malviya1, Marcelo Ganzella1
1Laboratory of Neurobiology, Max-Planck-Institute for Multidisciplinary Sciences, 37077 Göttingen, Germany.
The Journal of Biological Chemistry
|July 22, 2026
Summary
Researchers investigated the solute carrier SLC17A9
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Solute carrier SLC17A9 was identified as a vesicular nucleotide transporter.
- Its deletion in mice led to ATP loss from intracellular storage organelles.
Purpose of the Study:
- To characterize the molecular features of SLC17A9's transport activity.
- To investigate its potential role as a sodium-dependent phosphate transporter.
Main Methods:
- Functional reconstitution of purified recombinant SLC17A9 in liposomes.
- Assessing nucleotide transport activity under intact electrochemical potential.
- Testing phosphate transport activity via expression in HeLa cells.
Main Results:
- Purified SLC17A9, despite proper folding and orientation, showed no detectable nucleotide transport activity.
- No Na+-dependent phosphate transport activity was observed.
- HeLa cell expression did not consistently increase phosphate uptake.
Conclusions:
- The proposed role of SLC17A9 in vesicular nucleotide transport requires further validation.
- Additional, unknown factors may be necessary for SLC17A9 function.
- The transporter's function remains largely uncharacterized.
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