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Published on: April 30, 2019
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Multiplexed Imaging Strategy to Distinguish Indeterminant Biliary Strictures: An Ex Vivo Study.
M B Sturm1, B P Joshi1, S R Owens2
1Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, 48109, USA.
World Journal of Gastroenterology, Hepatology and Endoscopy
|November 8, 2022
Summary
Fluorescently-labeled peptides can differentiate cancerous biliary neoplasia from normal tissue. This multiplexed imaging approach may improve diagnosis of indeterminate biliary strictures.
Area of Science:
- Gastroenterology
- Oncology
- Biotechnology
Background:
- Indeterminate biliary strictures pose diagnostic challenges, being either malignant or benign.
- Biliary intraepithelial neoplasia (BilIN) is a precursor to cholangiocarcinoma, a lethal bile duct cancer.
- Current diagnostic methods are limited by insufficient cell and tissue sample sizes.
Purpose of the Study:
- To demonstrate the use of fluorescently-labeled peptides for multiplexed imaging of biliary neoplasia.
- To target specific biomarkers including EGFR, claudin-1, and ErbB2.
Main Methods:
- Human biliary stricture specimens were sectioned and stained.
- Fluorescently-labeled peptides (FITC, Cy5, IRDye800) targeting EGFR, claudin-1, and ErbB2 were used.
- Confocal microscopy was employed for multiplexed immunofluorescence imaging.
Main Results:
- Peptide binding was observed on the surface of biliary duct epithelial cells.
- Significantly greater fluorescence intensity was detected in neoplastic tissue compared to normal epithelium (ratios 1.4-1.6).
- Peptide and antibody binding showed strong co-localization (correlations of ρ=0.64, 0.51, 0.62).
Conclusions:
- A panel of fluorescently-labeled peptides can distinguish biliary neoplasia from normal epithelium.
- This method shows potential for multiplexed imaging in diagnosing indeterminate biliary strictures.
- The peptide-based approach may overcome limitations of current diagnostic techniques.
